Modeling Hypoxic Stress In Vitro Using Human Embryonic Stem Cells Derived Cardiomyocytes Matured by FGF4 and Ascorbic Acid Treatment
- Authors
- Choi, Seung-Cheol; Seo, Ha-Rim; Cui, Long-Hui; Song, Myeong-Hwa; Noh, Ji-Min; Kim, Kyung-Seob; Choi, Ji-Hyun; Kim, Jong-Ho; Park, Chi-Yeon; Joo, Hyung Joon; Hong, Soon Jun; Ko, Tae Hee; Choi, Jong-Il; Kim, Hyo Jin; Kim, Jong-Hoon; Paek, Se-Hwan; Park, Ji-Na; Kim, Dong-Hyung; Jang, Yongjun; Park, Yongdoo; Lim, Do-Sun
- Issue Date
- Oct-2021
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Keywords
- pluripotent stem cells; cytokines; cardiac; differentiation; hypoxia
- Citation
- Cells, v.10, no.10
- Indexed
- SCIE
SCOPUS
- Journal Title
- Cells
- Volume
- 10
- Number
- 10
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/54744
- DOI
- 10.3390/cells10102741
- ISSN
- 2073-4409
2073-4409
- Abstract
- Mature cardiomyocytes (CMs) obtained from human pluripotent stem cells (hPSCs) have been required for more accurate in vitro modeling of adult-onset cardiac disease and drug discovery. Here, we found that FGF4 and ascorbic acid (AA) induce differentiation of BG01 human embryonic stem cell-cardiogenic mesoderm cells (hESC-CMCs) into mature and ventricular CMs. Co-treatment of BG01 hESC-CMCs with FGF4+AA synergistically induced differentiation into mature and ventricular CMs. FGF4+AA-treated BG01 hESC-CMs robustly released acute myocardial infarction (AMI) biomarkers (cTnI, CK-MB, and myoglobin) into culture medium in response to hypoxic injury. Hypoxia-responsive genes and potential cardiac biomarkers proved in the diagnosis and prognosis of coronary artery diseases were induced in FGF4+AA-treated BG01 hESC-CMs in response to hypoxia based on transcriptome analyses. This study demonstrates that it is feasible to model hypoxic stress in vitro using hESC-CMs matured by soluble factors.</p>
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- Appears in
Collections - 1. Basic Science > Department of Biomedical Engineering > 1. Journal Articles
- 2. Clinical Science > Department of Cardiology > 1. Journal Articles
- 3. Graduate School > Graduate School > 1. Journal Articles
- 4. Research institute > Cardiovascular Research Institute > 1. Journal Articles
- 4. Research institute > Metabolic Syndrome Research Center > 1. Journal Articles
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