Discovery of Novel Pseudomonas putida Flavin-Binding Fluorescent Protein Variants with Significantly Improved Quantum Yield
- Authors
- Ko, Sanghwan; Jeon, Hyunwoo; Yoon, Sanghan; Kyung, Munsu; Yun, Hyungdon; Na, Jung-Hyun; Jung, Sang Taek
- Issue Date
- May-2020
- Publisher
- American Chemical Society
- Keywords
- flavin-binding fluorescent protein; fluorescence intensity; quantum yield
- Citation
- Journal of Agricultural and Food Chemistry, v.68, no.21, pp 5873 - 5879
- Pages
- 7
- Indexed
- SCIE
SCOPUS
- Journal Title
- Journal of Agricultural and Food Chemistry
- Volume
- 68
- Number
- 21
- Start Page
- 5873
- End Page
- 5879
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/802
- DOI
- 10.1021/acs.jafc.0c00121
- ISSN
- 0021-8561
1520-5118
- Abstract
- Oxygen-independent, flavin-binding fluorescent proteins (FbFPs) are emerging as alternatives to green fluorescent protein (GFP), which has limited applicability in studying anaerobic microorganisms, such as human gastrointestinal bacteria, which grow in oxygen-deficient environments. However, the utility of these FbFPs has been compromised because of their poor fluorescence emission. To overcome this limitation, we have employed a high-throughput library screening strategy and engineered an FbFP derived from Pseudomonas putida (SB2) for enhanced quantum yield. Of the resulting SB2 variants, KOFP-7 exhibited a significantly improved quantum yield (0.61) compared to other reported engineered FbFPs, which was even higher than that of enhanced GFP (EGFP, 0.60), with significantly enhanced tolerance against a strong reducing agent.
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Collections - 3. Graduate School > Biomedical Research Center > 1. Journal Articles
- 4. Research institute > Institute of Human Genetics > 1. Journal Articles
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