Adipose gene expression profiles related to metabolic syndrome using microarray analyses in two different models
- Authors
- Yoo H.J.; Hwang H.-J.; Jung T.W.; Ryu J.Y.; Hong H.C.; Choi H.Y.; Baik S.H.; Choi K.M.
- Issue Date
- 2014
- Publisher
- Korean Diabetes Association
- Keywords
- Lipocalin-2; Microarray; PPAR gamma
- Citation
- Diabetes and Metabolism Journal, v.38, no.5, pp 356 - 365
- Pages
- 10
- Indexed
- SCOPUS
KCI
- Journal Title
- Diabetes and Metabolism Journal
- Volume
- 38
- Number
- 5
- Start Page
- 356
- End Page
- 365
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/9896
- DOI
- 10.4093/dmj.2014.38.5.356
- ISSN
- 2233-6079
2233-6087
- Abstract
- Background: Peroxisome proliferator-activated receptor-γ (PPAR-γ) agonist has a wide-ranging influence on multiple components of metabolic syndrome. The Otsuka Long-Evans Tokushima Fatty (OLETF) rat is a useful animal model of metabolic syndrome. To determine genes related to metabolic syndrome, we examined overlapping genes that are simultaneously decreased by PPAR-γ agonists and increased in OLETF rats using microarrays in two different models. Methods: In the first microarray analysis, PPAR-γ agonist-treated db/db mice were compared to standard diet-fed db/db mice. In the second microarray analysis, OLETF rats were compared to Long-Evans Tokushima Otsuka (LETO) rats (control of OLETF rats). Results: Among the overlapping genes, in the present study, we validated that lipocalin-2 expression was significantly decreased in the visceral adipose tissue of PPAR-γ agonist-treated db/db mice compared to standard diet-fed db/db mice and increased in OLETF rats compared to LETO rats using real time reverse transcription polymerase chain reaction. Furthermore, we showed for the first time that lipocalin-2 expression was significantly increased in the visceral adipose tissues of obese humans compared with nonobese humans. In addition, the expression level of lipocalin-2 in human visceral adipose tissue had a significant positive correlation with body mass index, serum interleukin-6, adipocyte fatty acid binding protein levels, and white blood cell count. Conclusion: Lipocalin-2 was confirmed to be a significant adipokine affected by PPAR-γ agonist and obesity in the present study. Also, for the first time in human visceral adipose tissue, it was determined that the expression of lipocalin-2 from obese humans was significantly increased and correlated with circulating inflammatory markers. © 2014 Korean Diabetes Association.
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Collections - 2. Clinical Science > Department of Endocrinology and Metabolism > 1. Journal Articles
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