Direct observation of ligand-induced receptor dimerization with a bioresponsive hydrogel
- Authors
- Kim, Jongseong; Park, Yongdoo; Brown, Ashley C.; Lyon, L. Andrew
- Issue Date
- Dec-2013
- Publisher
- Royal Society of Chemistry
- Citation
- RSC Advances, v.4, no.110, pp 65173 - 65175
- Pages
- 3
- Indexed
- SCIE
SCOPUS
- Journal Title
- RSC Advances
- Volume
- 4
- Number
- 110
- Start Page
- 65173
- End Page
- 65175
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/9994
- DOI
- 10.1039/c4ra13251c
- ISSN
- 2046-2069
2046-2069
- Abstract
- Multimerization of biomolecules is essential for biological function and thus there is a need for sensitive biochemical assays that determine whether a molecule associates with one or more other molecules in the context of biological function. In this contribution we demonstrate a simple yet versatile method for the identification of physiologically important receptor dimerization events induced by a ligand. Bioresponsive hydrogel microparticles (microgels) conjugated with a receptor, Glycoprotein Ib alpha (GPIb alpha), display large changes in optical (microscopic) appearance under conditions known for to promote thrombin-induced GPIb alpha dimerization. In support of X-ray crystal structures, we identify that one thrombin molecule associates with two GPIb alpha moieties, which may play a role in efficient hemostatic function by increasing local concentration of GPIba on platelet surfaces. This microgel assay could provide a new way of studying important physiological and pathological mechanisms related to receptor dimerization and/or clustering.
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- Appears in
Collections - 1. Basic Science > Department of Biomedical Engineering > 1. Journal Articles
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