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Cited 28 time in webofscience Cited 31 time in scopus
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Development of a regenerative porous PLCL nerve guidance conduit with swellable hydrogel-based microgrooved surface pattern via 3D printing

Authors
Lee, Hyun SuJeon, Eun YoungNam, Jae JunPark, Ji HunChoi, In CheulKim, Soo HyunChung, Justin J.Lee, KangwonPark, Jong WoongJung, Youngmee
Issue Date
Mar-2022
Publisher
Elsevier BV
Keywords
PLCL nanoporous membrane; Visible light-crosslinked gelatin hydrogel; 3D printing; Nerve guidance conduit; Peripheral nerve regeneration
Citation
Acta Biomaterialia, v.141, pp 219 - 232
Pages
14
Indexed
SCIE
SCOPUS
Journal Title
Acta Biomaterialia
Volume
141
Start Page
219
End Page
232
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/55586
DOI
10.1016/j.actbio.2022.01.042
ISSN
1742-7061
1878-7568
Abstract
Peripheral nerve injury causes severe loss of motor and sensory functions, consequently increasing morbidity in affected patients. An autogenous nerve graft is considered the current gold standard for reconstructing nerve defects and recovering lost neurological functions; however, there are certain limitations to this method, such as limited donor nerve supply. With advances in regenerative medicine, recent research has focused on the fabrication of tissue-engineered nerve grafts as promising alternatives to the autogenous nerve grafts. In this study, we designed a nerve guidance conduit using an electrospun poly(lactide-co-epsilon-caprolactone) (PLCL) membrane with a visible light-crosslinked gelatin hydrogel. The PLCL nanoporous membrane with permeability served as a flexible and non-collapsible epineurium for the nerve conduit; the inner-aligned gelatin hydrogel paths were fabricated via 3D printing and a photocrosslinking system. The resultant gelatin hydrogel with microgrooved surface pattern was established as a conducting guidance path for the effective regeneration of axons and served as a reservoir that can incorporate and release bioactive molecules. From in vivo performance tests using a rat sciatic nerve defect model, our PLCL/gelatin conduit demonstrated successful axonal regeneration, remyelination capacities and facilitated functional recovery. Hence, the PLCL/gelatin conduit developed in this study is a promising substitute for autogenous nerve grafts. Statement of significance Nerve guidance conduits (NGCs) are developed as promising recovery techniques for bridging peripheral nerve defects. However, there are still technological limitations including differences in the structures and components between natural peripheral nerve and NGCs. In this study, we designed a NGC composed of an electrospun poly(lactide-co-epsilon-caprolactone) (PLCL) membrane and 3D printed inner gelatin hydrogel to serve as a flexible and non-collapsible epineurium and a conducting guidance path, respectively, to mimic the fascicular structure of the peripheral nerve. In particular , in vitro cell tests clearly showed that gelatin hydrogel could guide the cells and function as a reservoir that incorporate and release nerve growth factor. From in vivo performance tests, our regenerative conduit successfully led to axonal regeneration with effective functional recovery.
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Researcher Choi, In Cheul photo

Choi, In Cheul
Anam Hospital (Department of Orthopedic Surgery, Anam Hospital)
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