Submolecular Ligand Size and Spacing for Cell Adhesion
- Kim, Yuri; Koo, Thomas Myeongseok; Thangam, Ramar; Kim, Myeong Soo; Jang, Woo Young; Kang, Nayeon; Min, Sunhong; Kim, Seong Yeol; Yang, Letao; Hong, Hyunsik; Jung, Hee Joon; Koh, Eui Kwan; Patel, Kapil D.; Lee, Sungkyu; Fu, Hong En; Jeon, Yoo Sang; Park, Bum Chul; Kim, Soo Young; Park, Steve; Lee, Junmin; Gu, Luo; Kim, Dong-Hyun; Kim, Tae-Hyung; Lee, Ki-Bum; Jeong, Woong Kyo; Paulmurugan, Ramasamy; Kim, Young Keun; Kang, Heemin
- Issue Date
- WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
- cell adhesion; ligand size; ligand spacing; macrophage modulation; nanoassemblies; submolecular ligands
- Advanced Materials, v.34, no.27
- Journal Title
- Advanced Materials
- Cell adhesion occurs when integrin recognizes and binds to Arg-Gly-Asp (RGD) ligands present in fibronectin. In this work, submolecular ligand size and spacing are tuned via template-mediated in situ growth of nanoparticles for dynamic macrophage modulation. To tune liganded gold nanoparticle (GNP) size and spacing from 3 to 20 nm, in situ localized assemblies of GNP arrays on nanomagnetite templates are engineered. 3 nm-spaced ligands stimulate the binding of integrin, which mediates macrophage-adhesion-assisted pro-regenerative polarization as compared to 20 nm-spaced ligands, which can be dynamically anchored to the substrate for stabilizing integrin binding and facilitating dynamic macrophage adhesion. Increasing the ligand size from 7 to 20 nm only slightly promotes macrophage adhesion, not observed with 13 nm-sized ligands. Increasing the ligand spacing from 3 to 17 nm significantly hinders macrophage adhesion that induces inflammatory polarization. Submolecular tuning of ligand spacing can dominantly modulate host macrophages.
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- 2. Clinical Science > Department of Orthopedic Surgery > 1. Journal Articles
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