Whole structural reconstruction and quantification of epidermal innervation through the suction blister method and skin-clearing techniqueopen access
- Authors
- Kim, Dai Hyun; Lee, Se Jeong; Kim, June Hoan; Park, Sung Jin; Seo, Soo Hong; Ahn, Hyo Hyun; Sun, Woong; Kim, Byung-Jo; Rhyu, Im Joo
- Issue Date
- Sep-2022
- Publisher
- Nature Publishing Group
- Citation
- Scientific Reports, v.12, no.1
- Indexed
- SCIE
SCOPUS
- Journal Title
- Scientific Reports
- Volume
- 12
- Number
- 1
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/61465
- DOI
- 10.1038/s41598-022-16986-7
- ISSN
- 2045-2322
2045-2322
- Abstract
- Three-dimensional (3-D) analysis of intraepidermal nerve fibers (IENFs) is conducted to advance assessment methods for peripheral neuropathies and pruritic skin disorders. The skin-clearing technique was proven to be a reliable method for 3-D imaging of IENFs. Nonetheless, it still requires further improvement in the imaging process. The aim of this study was to standardize the 3-D evaluation method of IENFs and to suggest promising 3-D biomarkers for clinical application. A total of nine healthy individuals were prospectively enrolled. The newly adopted suction blister method was combined with the tissue-clearing technique. The detailed structure of the IENFs was reconstructed and quantified using the neuron tracing software. The suction blister method showed improved linear integrity of IENFs compared with those obtained from the previously used salt-split skin test. The 3-D parameters most significantly related to natural aging were the convex hull two-dimensional perimeter and the total length (both p = 0.020). The meaningful correlations were followed by total volume (p = 0.025), ends (p = 0.026), convex hull 3-D surface, and complexity (both p = 0.030). Thus, the 3-D parameters could be utilized as possible biomarkers to identify ambiguous pathologies of peripheral neuropathies and pruritic skin disorders.
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- Appears in
Collections - 2. Clinical Science > Department of Neurology > 1. Journal Articles
- 1. Basic Science > Department of Anatomy > 1. Journal Articles
- 4. Research institute > Practical Anatomy Research Institute > 1. Journal Articles
- 2. Clinical Science > Department of Dermatology > 1. Journal Articles
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