Interleukin-10 Knockout Mice Do Not Reliably Exhibit Macroscopic Inflammation: A Natural History Endoscopic Surveillance Study
- Authors
- Kim, Seung Young; Park, Jae Ho; Leite, Gabriela; Pimentel, Mark; Rezaie, Ali
- Issue Date
- Mar-2023
- Publisher
- Kluwer Academic/Plenum Publishers
- Keywords
- Colitis; IL-10 knockout mouse model; Endoscopy
- Citation
- Digestive Diseases and Sciences, v.68, no.5, pp 1858 - 1862
- Pages
- 5
- Indexed
- SCIE
SCOPUS
- Journal Title
- Digestive Diseases and Sciences
- Volume
- 68
- Number
- 5
- Start Page
- 1858
- End Page
- 1862
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/62893
- DOI
- 10.1007/s10620-023-07871-y
- ISSN
- 0163-2116
1573-2568
- Abstract
- Background
Interleukin (IL)-10 knockout (KO) mice, a model for inflammatory bowel disease (IBD), develop chronic enterocolitis due to an aberrant immune response to enteric antigens. Endoscopy, the gold standard for evaluation of human mucosal health, is not widely available for murine models.
Aims
To assess the natural history of left-sided colitis in IL-10 KO mice via serial endoscopies.
Methods
BALB/cJ IL-10 KO mice underwent regular endoscopic assessments from 2 up to 8 months of age. Procedures were recorded and blindly evaluated using a 4-component endoscopic score: mucosal wall transparency, intestinal bleeding, focal lesions and perianal lesions (0–3 points each). An endoscopic score ≥ 1 point was considered as the presence of colitis/flare.
Results
IL-10 KO mice (N = 40, 9 female) were assessed. Mean age at first endoscopy was 62.5 ± 2.5 days; average number of procedures per mouse was 6.0 ± 1.3. A total of 238 endoscopies were conducted every 24.8 ± 8.3 days, corresponding to 124.1 ± 45.2 days of surveillance per mouse. Thirty-three endoscopies in 24 mice (60%) detected colitis, mean endoscopy score 2.5 ± 1.3 (range: 1–6.3). Nineteen mice (47.5%) had one episode of colitis and 5 (12.5%) had 2–3 episodes. All exhibited complete spontaneous healing on subsequent endoscopies.
Conclusions
In this large-scale endoscopic surveillance study of IL-10 KO mice, 40% of mice did not develop endoscopic left-sided colitis. Furthermore, IL-10 KO mice did not exhibit persistent colitis and universally exhibited complete spontaneous healing without treatment. The natural history of colitis in IL-10 KO mice may not be comparable with that of IBD in humans and requires careful consideration.
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- Appears in
Collections - 2. Clinical Science > Department of Gastroenterology and Hepatology > 1. Journal Articles
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