Separation and Washing of Candida Cells from White Blood Cells Using Viscoelastic Microfluidicsopen access
- Authors
- Lim, Hyunjung; Kim, Jae Young; Choo, Seunghee; Lee, Changseok; Han, Byoung Joe; Lim, Chae Seung; Nam, Jeonghun
- Issue Date
- Apr-2023
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Keywords
- Candida; white blood cell; separation; washing; viscoelastic fluid
- Citation
- Micromachines, v.14, no.4
- Indexed
- SCIE
SCOPUS
- Journal Title
- Micromachines
- Volume
- 14
- Number
- 4
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/62896
- DOI
- 10.3390/mi14040712
- ISSN
- 2072-666X
2072-666X
- Abstract
- An early and accurate diagnosis of Candida albicans is critical for the rapid antifungal treatment of candidemia, a mortal bloodstream infection. This study demonstrates viscoelastic microfluidic techniques for continuous separation, concentration, and subsequent washing of Candida cells in the blood. The total sample preparation system contains two-step microfluidic devices: a closed-loop separation and concentration device and a co-flow cell-washing device. To determine the flow conditions of the closed-loop device, such as the flow rate factor, a mixture of 4 and 13 μm particles was used. Candida cells were successfully separated from the white blood cells (WBCs) and concentrated by 74.6-fold in the sample reservoir of the closed-loop system at 800 μL/min with a flow rate factor of 3.3. In addition, the collected Candida cells were washed with washing buffer (deionized water) in the microchannels with an aspect ratio of 2 at a total flow rate of 100 μL/min. Finally, Candida cells at extremely low concentrations (Ct > 35) became detectable after the removal of WBCs, the additional buffer solution in the closed-loop system (Ct = 30.3 ± 1.3), and further removal of blood lysate and washing (Ct = 23.3 ± 1.6).
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- Appears in
Collections - 2. Clinical Science > Department of Laboratory Medicine > 1. Journal Articles
- 4. Research institute > Research Institute for Skin Image > 1. Journal Articles
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