Comparative Clinical Evaluation of a Novel FluA/FluB/SARS-CoV-2 Multiplex LAMP and Commercial FluA/FluB/SARS-CoV-2/RSV RT-qPCR Assaysopen access
- Authors
- Jee, Hyunseul; Park, Seoyeon; Lee, Junmin; Lim, Chae Seung; Jang, Woong Sik
- Issue Date
- Apr-2023
- Publisher
- MDPI AG
- Keywords
- SARS-CoV-2; influenza A; influenza B; loop-mediated isothermal amplification (LAMP); multiplex LAMP
- Citation
- Diagnostics, v.13, no.8
- Indexed
- SCIE
SCOPUS
- Journal Title
- Diagnostics
- Volume
- 13
- Number
- 8
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/63244
- DOI
- 10.3390/diagnostics13081432
- ISSN
- 2075-4418
- Abstract
- Influenza and coronaviruses cause highly contagious respiratory diseases that cause millions of deaths worldwide. Public health measures implemented during the current coronavirus disease (COVID-19) pandemic have gradually reduced influenza circulation worldwide. As COVID-19 measures have relaxed, it is necessary to monitor and control seasonal influenza during this COVID-19 pandemic. In particular, the development of rapid and accurate diagnostic methods for influenza and COVID-19 is of paramount importance because both diseases have significant public health and economic impacts. To address this, we developed a multi-loop-mediated isothermal amplification (LAMP) kit capable of simultaneously detecting influenza A/B and SARS-CoV-2. The kit was optimized by testing various ratios of primer sets for influenza A/B (FluA/FluB) and SARS-CoV-2 and internal control (IC). The FluA/FluB/SARS-CoV-2 multiplex LAMP assay showed 100% specificity for uninfected clinical samples and sensitivities of 90.6%, 86.89%, and 98.96% for LAMP kits against influenza A, influenza B, and SARS-CoV-2 clinical samples, respectively. Finally, the attribute agreement analysis for clinical tests indicated substantial agreement between the multiplex FluA/FluB/SARS-CoV-2/IC LAMP and commercial Allplex (TM) SARS-CoV-2/FluA/FluB/RSV assays.
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Collections - 4. Research institute > Institute for Trauma Research > 1. Journal Articles
- 2. Clinical Science > Department of Laboratory Medicine > 1. Journal Articles
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