Fusion Protein of RBP and Albumin Domain III Reduces Lung Fibrosis by Inactivating Lung Stellate Cellsopen access
- Authors
- Choi, Jaeho; Son, Yuna; Moon, Ji Wook; Park, Dae Won; Kim, Young-Sik; Oh, Junseo
- Issue Date
- Jul-2023
- Publisher
- MDPI AG
- Keywords
- lung fibrosis; stellate cells; R-III
- Citation
- Biomedicines, v.11, no.7
- Indexed
- SCIE
SCOPUS
- Journal Title
- Biomedicines
- Volume
- 11
- Number
- 7
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/63765
- DOI
- 10.3390/biomedicines11072007
- ISSN
- 2227-9059
2227-9059
- Abstract
- Activated stellate cells play a role in fibrosis development in the liver, pancreas, and kidneys. The fusion protein R-III, which consists of retinol-binding protein and albumin domain III, has been demonstrated to attenuate liver and renal fibrosis by suppressing stellate cell activation. In this study, we investigated the efficacy of R-III against bleomycin-induced lung fibrosis in mice. R-III reduced lung fibrosis and primarily localized in autofluorescent cells in the lung tissue. Furthermore, we isolated lung stellate cells (LSCs) from rat lungs using the isolation protocol employed for hepatic stellate cells (HSCs). LSCs shared many characteristics with HSCs, including the presence of vitamin A-containing lipid droplets and the expression of alpha-smooth muscle actin and collagen type I, markers for activated HSCs/myofibroblasts. LSCs spontaneously transdifferentiated into myofibroblasts in in vitro culture, which was inhibited by R-III. These findings suggest that R-III may reduce lung fibrosis by inactivating LSCs and could be a promising treatment for extrahepatic fibrosis.
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- Appears in
Collections - 3. Graduate School > Biomedical Research Center > 1. Journal Articles
- 2. Clinical Science > Department of Pathology > 1. Journal Articles
- 2. Clinical Science > Department of Infectious Diseases > 1. Journal Articles
- 3. Graduate School > Graduate School > 1. Journal Articles
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