Factors affecting the cleavage efficiency of the CRISPR-Cas9 system
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung, Won Jun | - |
dc.contributor.author | Park, Soo-Ji | - |
dc.contributor.author | Cha, Seongkwang | - |
dc.contributor.author | Kim, Kyoungmi | - |
dc.date.accessioned | 2024-03-27T01:30:09Z | - |
dc.date.available | 2024-03-27T01:30:09Z | - |
dc.date.issued | 2024-03 | - |
dc.identifier.issn | 1976-8354 | - |
dc.identifier.issn | 2151-2485 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/65789 | - |
dc.description.abstract | The CRISPR-Cas system stands out as a promising genome editing tool due to its cost-effectiveness and time efficiency compared to other methods. This system has tremendous potential for treating various diseases, including genetic disorders and cancer, and promotes therapeutic research for a wide range of genetic diseases. Additionally, the CRISPR-Cas system simplifies the generation of animal models, offering a more accessible alternative to traditional methods. The CRISPR-Cas9 system can be used to cleave target DNA strands that need to be corrected, causing double-strand breaks (DSBs). DNA with DSBs can then be recovered by the DNA repair pathway that the CRISPR-Cas9 system uses to edit target gene sequences. High cleavage efficiency of the CRISPR-Cas9 system is thus imperative for effective gene editing. Herein, we explore several factors affecting the cleavage efficiency of the CRISPR-Cas9 system. These factors include the GC content of the protospacer-adjacent motif (PAM) proximal and distal regions, single-guide RNA (sgRNA) properties, and chromatin state. These considerations contribute to the efficiency of genome editing. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 한국통합생물학회 | - |
dc.title | Factors affecting the cleavage efficiency of the CRISPR-Cas9 system | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.1080/19768354.2024.2322054 | - |
dc.identifier.scopusid | 2-s2.0-85186444734 | - |
dc.identifier.wosid | 001177052400001 | - |
dc.identifier.bibliographicCitation | Animal Cells and Systems, v.28, no.1, pp 75 - 83 | - |
dc.citation.title | Animal Cells and Systems | - |
dc.citation.volume | 28 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 75 | - |
dc.citation.endPage | 83 | - |
dc.type.docType | Review | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Zoology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Zoology | - |
dc.subject.keywordPlus | STRAND BREAK REPAIR | - |
dc.subject.keywordPlus | GENOME MODIFICATION | - |
dc.subject.keywordPlus | IMMUNE-SYSTEM | - |
dc.subject.keywordPlus | CRISPR/CAS9 | - |
dc.subject.keywordPlus | CAS9 | - |
dc.subject.keywordPlus | NUCLEASES | - |
dc.subject.keywordPlus | TOOL | - |
dc.subject.keywordAuthor | CRISPR-Cas9 system | - |
dc.subject.keywordAuthor | genome editing | - |
dc.subject.keywordAuthor | cleavage efficiency | - |
dc.subject.keywordAuthor | sgRNA | - |
dc.subject.keywordAuthor | chromatin state | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
73, Goryeodae-ro, Seongbuk-gu, Seoul, Republic of Korea (02841)82-2-2286-1265
COPYRIGHT 2020 KOREA UNIVERSITY MEDICAL LIBRARY ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.