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Factors affecting the cleavage efficiency of the CRISPR-Cas9 systemopen access

Authors
Jung, Won JunPark, Soo-JiCha, SeongkwangKim, Kyoungmi
Issue Date
Dec-2024
Publisher
한국통합생물학회
Keywords
CRISPR-Cas9 system; genome editing; cleavage efficiency; sgRNA; chromatin state
Citation
Animal Cells and Systems, v.28, no.1, pp 75 - 83
Pages
9
Indexed
SCIE
SCOPUS
KCI
Journal Title
Animal Cells and Systems
Volume
28
Number
1
Start Page
75
End Page
83
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/65789
DOI
10.1080/19768354.2024.2322054
ISSN
1976-8354
2151-2485
Abstract
The CRISPR-Cas system stands out as a promising genome editing tool due to its cost-effectiveness and time efficiency compared to other methods. This system has tremendous potential for treating various diseases, including genetic disorders and cancer, and promotes therapeutic research for a wide range of genetic diseases. Additionally, the CRISPR-Cas system simplifies the generation of animal models, offering a more accessible alternative to traditional methods. The CRISPR-Cas9 system can be used to cleave target DNA strands that need to be corrected, causing double-strand breaks (DSBs). DNA with DSBs can then be recovered by the DNA repair pathway that the CRISPR-Cas9 system uses to edit target gene sequences. High cleavage efficiency of the CRISPR-Cas9 system is thus imperative for effective gene editing. Herein, we explore several factors affecting the cleavage efficiency of the CRISPR-Cas9 system. These factors include the GC content of the protospacer-adjacent motif (PAM) proximal and distal regions, single-guide RNA (sgRNA) properties, and chromatin state. These considerations contribute to the efficiency of genome editing.
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1. Basic Science > Department of Physiology > 1. Journal Articles
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