High-resolution phylogeographical surveillance of Hantaan orthohantavirus using rapid amplicon-based Flongle sequencing, Republic of Korea
- Authors
- Kim, Jongwoo; Park, Kyungmin; Kim, Kijin; Noh, Juyoung; Kim, Seong-Gyu; Yang, Eunyoung; Cho, Hee-Kyung; Lee, Seung-Ho; No, Jin Sun; Lee, Geum-Young; Lee, Daesang; Song, Dong-Hyun; Gu, Se Hun; Park, Man-Seong; Cho, Nam-Hyuk; Jeong, Seong Tae; Kim, Won-Keun; Song, Jin-Won
- Issue Date
- Jan-2024
- Publisher
- John Wiley & Sons Inc.
- Keywords
- evolutionary dynamics; Flongle sequencing; Hantaan orthohantavirus; hemorrhagic fever with renal syndrome; phylogeographical surveillance
- Citation
- Journal of Medical Virology, v.96, no.1
- Indexed
- SCIE
SCOPUS
- Journal Title
- Journal of Medical Virology
- Volume
- 96
- Number
- 1
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/65860
- DOI
- 10.1002/jmv.29346
- ISSN
- 0146-6615
1096-9071
- Abstract
- Orthohantaviruses, etiological agents of hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome, pose a critical public health threat worldwide. Hantaan orthohantavirus (HTNV) outbreaks are particularly endemic in Gyeonggi Province in northern area of the Republic of Korea (ROK). Small mammals were collected from three regions in the Gyeonggi Province during 2017 and 2018. Serological and molecular prevalence of HTNV was 25/201 (12.4%) and 10/25 (40%), respectively. A novel nanopore-based diagnostic assay using a cost-efficient Flongle chip was developed to rapidly and sensitively detect HTNV infection in rodent specimens within 3 h. A rapid phylogeographical surveillance of HTNV at high-resolution phylogeny was established using the amplicon-based Flongle sequencing. In total, seven whole-genome sequences of HTNV were newly obtained from wild rodents collected in Paju-si (Gaekhyeon-ri) and Yeoncheon-gun (Hyeonga-ri and Wangnim-ri), Gyeonggi Province. Phylogenetic analyses revealed well-supported evolutionary divergence and genetic diversity, enhancing the resolution of the phylogeographic map of orthohantaviruses in the ROK. Incongruences in phylogenetic patterns were identified among HTNV tripartite genomes, suggesting differential evolution for each segment. These findings provide crucial insights into on-site diagnostics, genome-based surveillance, and the evolutionary dynamics of orthohantaviruses to mitigate hantaviral outbreaks in HFRS-endemic areas in the ROK.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 1. Basic Science > Department of Microbiology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.