Peripheral mGluR5 antagonist attenuated craniofacial muscle pain and inflammation but not mGluR1 antagonist in lightly anesthetized rats
- Authors
- Lee, Ho Jeong; Choi, Hyo Soon; Ju, Jin Sook; Bae, Yong Chul; Kim, Sung Kyo; Yoon, Young Wook; Ahn, Dong Kuk
- Issue Date
- Oct-2006
- Publisher
- Elsevier BV
- Keywords
- antinociception; muscle inflammation; muscle pain; nociceptive behaviour; peripheral mGluR
- Citation
- Brain Research Bulletin, v.70, no.4-6, pp 378 - 385
- Pages
- 8
- Indexed
- SCOPUS
- Journal Title
- Brain Research Bulletin
- Volume
- 70
- Number
- 4-6
- Start Page
- 378
- End Page
- 385
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/18577
- DOI
- 10.1016/j.brainresbull.2005.09.021
- ISSN
- 0361-9230
1873-2747
- Abstract
- The present study investigated the role of peripheral group I metabotropic glutamate receptors (mGluRs) in MO-induced nociceptive behaviour and inflammation in the masseter muscles of lightly anesthetized rats. Experiments were carried out on male Sprague-Dawley rats weighing 300-400 g. After initial anesthesia with sodium pentobarbital (40 mg/kg, i.p.), one femoral vein was cannulated and connected to an infusion pump for intravenous infusion of sodium pentobarbital. The rate of infusion was adjusted to provide a constant level of anesthesia. Mustard oil (MO, 30 mu l) was injected into the mid-region of the left masseter muscle via a 30-gauge needle over 10 s. After 30 mu l injection of 5, 10, 15, or 20% MO into the masseter muscle, the total number of hindpaw shaking behaviour and extravasated Evans' blue dye concentration in the masseter muscle were significantly higher in the MO-treated group in a dose-dependent manner compared with the vehicle (mineral oil)-treated group. Intramuscular pretreatment with 3 or 5% lidocaine reduced MO-induced hindpaw shaking behaviour and increases in extravasated Evans' blue dye concentration. Intramuscular pretreatment with 5 mM MCPG, non-selective group I/II mGluR antagonist, or MPEP, a selective group I mGluR5 antagonist, produced a significant attenuation of MO-induced hindpaw shaking behaviour and increases in extravasated Evans' blue dye concentration in the masseter muscle while LY367385, a selective group I mGluR1 antagonist, did not affect MO-induced nociceptive behaviour and inflammation in the masseter muscle. These results indicate that peripheral mGluR5 plays important role in mediating MO-induced nociceptive behaviour and inflammation in the craniofacial muscle. (c) 2006 Elsevier Inc. All rights reserved.
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Collections - 1. Basic Science > Department of Physiology > 1. Journal Articles
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