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Cited 21 time in webofscience Cited 21 time in scopus
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Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement

Authors
Seo, Ha-RimJoo, Hyung JoonKim, Dae HwanCui, Long-HuiChoi, Seung-CheolKim, Jong-HoCho, Sung WooLee, Kyu BackLim, Do-Sun
Issue Date
24-May-2017
Publisher
AMER CHEMICAL SOC
Keywords
Flk1-positive mesodermal precursor cells; gradient nanopattern plates; cardiomyocyte differentiation; cytoskeleton reorganization; nanoimprintiug
Citation
ACS APPLIED MATERIALS & INTERFACES, v.9, no.20, pp.16804 - 16813
Indexed
SCIE
SCOPUS
Journal Title
ACS APPLIED MATERIALS & INTERFACES
Volume
9
Number
20
Start Page
16804
End Page
16813
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/4982
DOI
10.1021/acsami.7b01555
ISSN
1944-8244
Abstract
Nanoscaled surface patterning is an emerging potential method of directing the fate of stem cells. We adopted nanoscaled pillar gradient patterned cell culture plates with three diameter gradients [280-360 (GP 280/360), 200-280 (GP 200/280), and 120-200 nm (GP 120/200)] and investigated their cell fate-modifying effect on multipotent fetal liver kinase 1-positive mesodermal precursor cells (Flk1(+) MPCs) derived from embryonic stem cells. We observed increased cell proliferation and colony formation of the Flk1(+). MPCs on the nanopattern plates. Interestingly, the 200-280 nm-sized (GP 200/280) pillar surface dramatically increased cardiomyocyte differentiation and expression of the early cardiac marker gene Mesp1. The gradient nanopattern surface-induced cardiomyocytes had cardiac sarcomeres, With mature cardiac gene expression. We observed Vinculin and p-Cofilin-mediated cytoskeleton reorganization during this process. In summary, the gradient nanopattern surface with 200-280 nm-sized enhanced cardiomyocyte differentiation in Flk1(+) MPCs.
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4. Research institute > Cardiovascular Research Institute > 1. Journal Articles
4. Research institute > Metabolic Syndrome Research Center > 1. Journal Articles
2. Clinical Science > Department of Cardiology > 1. Journal Articles

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Joo, Hyung Joon
Anam Hospital (Department of Cardiology, Anam Hospital)
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