Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement
- Authors
- Seo, Ha-Rim; Joo, Hyung Joon; Kim, Dae Hwan; Cui, Long-Hui; Choi, Seung-Cheol; Kim, Jong-Ho; Cho, Sung Woo; Lee, Kyu Back; Lim, Do-Sun
- Issue Date
- 24-May-2017
- Publisher
- AMER CHEMICAL SOC
- Keywords
- Flk1-positive mesodermal precursor cells; gradient nanopattern plates; cardiomyocyte differentiation; cytoskeleton reorganization; nanoimprintiug
- Citation
- ACS APPLIED MATERIALS & INTERFACES, v.9, no.20, pp 16804 - 16813
- Pages
- 10
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- ACS APPLIED MATERIALS & INTERFACES
- Volume
- 9
- Number
- 20
- Start Page
- 16804
- End Page
- 16813
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/4982
- DOI
- 10.1021/acsami.7b01555
- ISSN
- 1944-8244
1944-8252
- Abstract
- Nanoscaled surface patterning is an emerging potential method of directing the fate of stem cells. We adopted nanoscaled pillar gradient patterned cell culture plates with three diameter gradients [280-360 (GP 280/360), 200-280 (GP 200/280), and 120-200 nm (GP 120/200)] and investigated their cell fate-modifying effect on multipotent fetal liver kinase 1-positive mesodermal precursor cells (Flk1(+) MPCs) derived from embryonic stem cells. We observed increased cell proliferation and colony formation of the Flk1(+). MPCs on the nanopattern plates. Interestingly, the 200-280 nm-sized (GP 200/280) pillar surface dramatically increased cardiomyocyte differentiation and expression of the early cardiac marker gene Mesp1. The gradient nanopattern surface-induced cardiomyocytes had cardiac sarcomeres, With mature cardiac gene expression. We observed Vinculin and p-Cofilin-mediated cytoskeleton reorganization during this process. In summary, the gradient nanopattern surface with 200-280 nm-sized enhanced cardiomyocyte differentiation in Flk1(+) MPCs.
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- Appears in
Collections - 4. Research institute > Cardiovascular Research Institute > 1. Journal Articles
- 4. Research institute > Metabolic Syndrome Research Center > 1. Journal Articles
- 2. Clinical Science > Department of Cardiology > 1. Journal Articles
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