BMP-2 induces motility and invasiveness by promoting colon cancer stemness through STAT3 activation
- Authors
- Kim, Bo Ram; Oh, Sang Cheul; Lee, Dae-Hee; Kim, Jung Lim; Lee, Suk Young; Kang, Myoung Hee; Lee, Sun Il; Kang, Sanghee; Joung, Sung Yup; Min, Byung Wook
- Issue Date
- Dec-2015
- Publisher
- SAGE PUBLICATIONS LTD
- Keywords
- Colon cancer; Bone morphogenetic proteins; Cancer stem cells; Epithelial-mesenchymal transition; Signal transducer and activator of transcription 3
- Citation
- TUMOR BIOLOGY, v.36, no.12, pp 9475 - 9486
- Pages
- 12
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- TUMOR BIOLOGY
- Volume
- 36
- Number
- 12
- Start Page
- 9475
- End Page
- 9486
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/7255
- DOI
- 10.1007/s13277-015-3681-y
- ISSN
- 1010-4283
1423-0380
- Abstract
- Bone morphogenetic proteins (BMPs) have been involved in metastatic progression and tumorigenesis of many cancer types. However, it remains unclear how BMP-2 contributes to the initiation and development of these cancers. Here, we investigated the role of BMP-2 in colon cancer stem cell (CSC) development from colon cancer cells. We also determined the effects of BMP-2 on CSC development and epithelial-mesenchymal transition (EMT) in human colon cancer cell lines HCT-116 and SW620. We found that BMP-2 enhanced sphere formation of colon cancer cells without serum. Also, BMP-2-induced spheres displayed up-regulation of stemness markers (CD133+ and EpCAM+) and increased drug resistance, hallmarks of CSCs. Importantly, expression of EMT activators p-Smad1/5 and Snail and N-cadherin was increased in the spheres' cells, indicating that BMP-2 signaling might result in CSC self-renewal and EMT. Furthermore, siRNA-mediated knockdown of signal transducer and activator of transcription 3 (STAT3) in HCT-116 cells reversed BMP-2-induced EMT and stem cell formation. Taken together, our results suggest that the BMP-2 induced STAT3-mediated induction of colon cancer cell metastasis requires an EMT and/or changes in CSC markers.
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- Appears in
Collections - 4. Research institute > Institute of Convergence New Drug Development > 1. Journal Articles
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