Rapid Detection of Mycobacterium Tuberculosis Using a Novel Point-of-Care BZ TB/NTM NALF Assay: Integrating LAMP and LFIA Technologiesopen access
- Authors
- Kim, Ha Nui; Lee, Junmin; Yoon, Soo-Young; Jang, Woong Sik; Lim, Chae Seung
- Issue Date
- Apr-2023
- Publisher
- MDPI AG
- Keywords
- tuberculosis; TB; MTB; NALF
- Citation
- Diagnostics, v.13, no.8
- Indexed
- SCIE
SCOPUS
- Journal Title
- Diagnostics
- Volume
- 13
- Number
- 8
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/63243
- DOI
- 10.3390/diagnostics13081497
- ISSN
- 2075-4418
- Abstract
- Tuberculosis (TB) is one of the leading causes of infectious mortality from a single infectious agent, Mycobacterium tuberculosis (MTB). This study evaluated the performance of the newly developed BZ TB/NTM NALF assay, which integrated loop-mediated isothermal amplification and lateral flow immunochromatographic assay technologies, for the detection of MTB. A total of 80 MTB-positive samples and 115 MTB-negative samples were collected, all of which were confirmed by TB real-time PCR (RT-PCR) using either AdvanSure (TM) TB/NTM RT-PCR Kit or Xpert((R)) MTB/RIF Assay. The performance of the BZ TB/NTM NALF assay was evaluated by calculating its sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) in comparison to those of the RT-PCR methods. Compared to the RT-PCR, the sensitivity, specificity, PPV, and NPV of BZ TB/NTM NALF assay were 98.7%, 99.1%, 98.7%, and 99.1%, respectively. The concordance rate between BZ TB/NTM NALF and RT-PCR was 99.0%. Rapid and simple detection of MTB is essential for global case detection and further elimination of TB. The performance of the BZ TB/NTM NALF Assay is acceptable with a high concordance with RT-PCR, indicating that it is reliable for use in a low-resource environment.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 4. Research institute > Institute for Trauma Research > 1. Journal Articles
- 2. Clinical Science > Department of Laboratory Medicine > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.